【佳學基因檢測】MicroRNA-18a 通過HIF-1α 表達調節(jié)口腔鱗狀細胞癌細胞的轉移特性

品牌基因檢測在哪里做——時機窗口

開題評估知道《BMC Oral Health》在. 2022 Sep 5;22(1):378.發(fā)表了一篇題目為《MicroRNA-18a 通過 HIF-1α 表達調節(jié)口腔鱗狀細胞癌細胞的轉移特性》腫瘤靶向藥物治療基因檢測臨床研究文章。該研究由Shihyun Kim, Suyeon Park, Ji-Hyeon Oh, Sang Shin Lee, Yoon Lee, Jongho Choi 等完成。促進了腫瘤的正確治療與個性化用藥的發(fā)展，進一步強調了基因信息檢測與分析的重要性。

腫瘤靶向藥物及正確治療臨床研究內容關鍵詞：

HIF-1α,缺氧,入侵,微小RNA,移民,口腔鱗狀細胞癌

腫瘤靶向治療基因檢測臨床應用結果

口腔鱗狀細胞癌轉移風險與靶向藥物治療基因檢測背景：口腔鱗狀細胞癌的快速轉移與預后不良和高死亡率相關。然而，總口腔鱗狀細胞癌轉移的分子機制尚未有效闡明。盡管 microRNA (miRNA) 的失調表達在惡性腫瘤進展中具有關鍵作用，但 miRNA 在 總口腔鱗狀細胞癌進展中的生物學功能仍不清楚?？谇击[狀細胞癌轉移風險與靶向藥物治療基因檢測研究旨在探討 miRNA-18a 通過缺氧誘導因子 1α (HIF-1α) 在 總口腔鱗狀細胞癌轉移調控中的作用?？谇击[狀細胞癌轉移風險與靶向藥物治療基因檢測方法： miRNA-18a-5p (miRNA-18a) 在 總患者 (n = 39) 和在 總口腔鱗狀細胞癌細胞系（例如，YD-10B 和 HSC-2 細胞）中，使用定量實時聚合酶鏈反應進行分析。使用蛋白質印跡分析用 miRNA-18a 模擬物或與氯化鈷組合處理的 總口腔鱗狀細胞癌細胞中的 HIF-1α 蛋白表達。采用MTT法、EdU法和Transwell®插入系統(tǒng)分析了總生存期CC細胞的miRNA-18a表達依賴性增殖和侵襲能力?？谇击[狀細胞癌轉移風險與靶向藥物治療基因檢測結果：總生存期CC組織中miRNA-18a表達明顯低于鄰近正常組織。在 總口腔鱗狀細胞癌細胞系中，HIF-1α 表達通過 miRNA-18a 模擬處理顯著降低。此外，與僅用 miRNA-18a 模擬物處理的細胞相比，miRNA-18a 模擬物顯著降低 總口腔鱗狀細胞癌細胞的遷移和侵襲能力，而在缺氧條件下 HIF-1α 的過表達顯著提高了 總口腔鱗狀細胞癌細胞的遷移和侵襲能力?？谇击[狀細胞癌轉移風險與靶向藥物治療基因檢測結論：miRNA-18a對 HIF-1α 表達產生負面影響并抑制 總口腔鱗狀細胞癌的轉移，從而表明其作為 總口腔鱗狀細胞癌抗轉移策略的治療靶點的潛力。缺氧;入侵；微小RNA；移民;口腔鱗狀細胞癌。

腫瘤發(fā)生與反復轉移國際數(shù)據(jù)庫描述：

Background: Rapid metastasis of oral squamous cell carcinoma (OSCC) is associated with a poor prognosis and a high mortality rate. However, the molecular mechanisms underlying OSCC metastasis have not been fully elucidated. Although deregulated expression of microRNA (miRNA) has a crucial role in malignant cancer progression, the biological function of miRNA in OSCC progression remains unclear. This study aimed to investigate the function of miRNA-18a in OSCC metastatic regulation via hypoxia-inducible factor 1α (HIF-1α).Methods: miRNA-18a-5p (miRNA-18a) expressions in patients with OSCC (n = 39) and in OSCC cell lines (e.g., YD-10B and HSC-2 cells) were analyzed using quantitative real-time polymerase chain reaction. HIF-1α protein expressions in OSCC cells treated with miRNA-18a mimics or combined with cobalt chloride were analyzed using western blotting. The miRNA-18a expression-dependent proliferation and invasion abilities of OSCC cells were analyzed using MTT assay, EdU assay, and a Transwell® insert system.Results: miRNA-18a expression was significantly lower in OSCC tissue than in the adjacent normal tissue. In OSCC cell lines, HIF-1α expression was significantly decreased by miRNA-18a mimic treatment. Furthermore, the migration and invasion abilities of OSCC cells were significantly decreased by miRNA-18a mimics and significantly increased by the overexpression of HIF-1α under hypoxic conditions relative to those abilities in cells treated only with miRNA-18a mimics.Conclusions: miRNA-18a negatively affects HIF-1α expression and inhibits the metastasis of OSCC, thereby suggesting its potential as a therapeutic target for antimetastatic strategies in OSCC.Keywords: HIF-1α; Hypoxia; Invasion; MicroRNA; Migration; Oral squamous cell carcinoma.

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