【佳學基因檢測】CCDC137表達的預后意義及其與肝細胞癌免疫浸潤的關系

上海腫瘤基因檢測機構機會

參加學術會議時了解《Dis Markers》在.?2022 Aug 24;2022:5638675.發(fā)表了一篇題目為《CCDC137表達的預后意義及其與肝細胞癌免疫浸潤的關系》腫瘤靶向藥物治療基因檢測臨床研究文章。該研究由Lu Bai,?Zhao-Xu Yang,?Jian-Shan Liu,?De-Sheng Wang,?Heng-Chao Yu?等完成。促進了腫瘤的正確治療與個性化用藥的發(fā)展，進一步強調了基因信息檢測與分析的重要性。

腫瘤靶向藥物及正確治療臨床研究內容關鍵詞：

腫瘤靶向治療基因檢測臨床應用結果

在全球范圍內，肝細胞癌 (HCC) 是癌癥相關死亡的最常見原因之一。由于遠處轉移和反復，HCC患者的臨床結果仍然很差。近年來，越來越多的證據(jù)證實含有卷曲螺旋結構域（CCDC）的家族蛋白參與了多種疾病的進展。然而，尚未研究包含卷曲螺旋結構域的 137 (CCDC137) 在肝細胞癌 (HCC) 中的表達和臨床意義。在 TCGA-LIHC 中獲得了 3 級 mRNA 表達譜和臨床病理學數(shù)據(jù)。在 371 個 HCC 和 50 個非腫瘤樣本之間篩選了差異表達基因 (DEG)。在 HCC 患者中分析了 CCDC137 的預后價值。研究了 CCDC137 與癌癥免疫浸潤之間的相關性。本研究共獲得2897個DEG：2451個基因顯著上調，446個基因顯著下調。 KEGG 分析顯示這些 DEG 參與了腫瘤進展。在 2897 個 DEG 中，我們發(fā)現(xiàn)與非腫瘤標本相比，HCC 標本中 CCDC137 的表達明顯增加。高水平的 CCDC137 表達與晚期腫瘤分期和分級有關。此外，與 CCDC137 水平較低的患者相比，CCDC137 表達水平較高的患者的總生存期和無病生存期較短。 CCDC137 表達與幾種免疫細胞的浸潤水平呈正相關，例如 CD8 T 細胞和 Th2 細胞。最后，體外實驗證實CCDC137在HCC細胞中高表達，其敲低抑制了HCC細胞的增殖?？傊?，我們的研究結果表明，CCDC137 可用作 HCC 免疫浸潤和預后不良的生物標志物，這為 HCC 的潛在療法提供了新的見解。

腫瘤發(fā)生與反復轉移國際數(shù)據(jù)庫描述：

Globally, hepatocellular carcinoma (HCC) is one of the most common causes of cancer-associated mortalities. The clinical outcome of HCC patients remains poor due to distant metastasis and recurrence. In recent years, growing evidences have confirmed that the coiled-coil domain-containing (CCDC) family proteins are involved in the progression of several diseases. However, the expression and clinical significance of the coiled-coil domain-containing 137 (CCDC137) in hepatocellular carcinoma (HCC) have not been investigated. Level 3 mRNA expression profiles and clinicopathological data were obtained in TCGA-LIHC. Differentially expressed genes (DEGs) were screened between 371 HCC and 50 nontumor specimens. The prognostic value of CCDC137 was analyzed in HCC patients. The correlations between CCDC137 and cancer immune infiltrates were investigated. In this study, a total of 2897 DEGs were obtained: 2451 genes were significantly upregulated and 446 genes were significantly downregulated. KEGG assays revealed that these DEGs were involved in tumor progression. Among 2897 DEGs, we found that CCDC137 expression was distinctly increased in HCC specimens compared with nontumor specimens. A high level of CCDC137 expression was related to an advanced tumor stage and grade. Moreover, patients with higher levels of CCDC137 expression had a shorter overall survival and disease-free survival than patients with lower CCDC137 levels. CCDC137 expression was positively correlated with infiltrating levels of several immune cells, such as CD8 T cells and Th2 cells. Finally, in vitro experiments confirmed that CCDC137 expression was highly expressed in HCC cells, and its knockdown suppressed the proliferation of HCC cells. Taken together, our findings revealed that CCDC137 might be used as a biomarker for immune infiltration and poor prognosis in HCC, which offered fresh insight on potential therapies for HCC.

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