【佳學(xué)基因檢測(cè)】口腔腫瘤細(xì)胞外泌體 miR-10b 基因檢測(cè)分析靶向藥物選擇

靶向基因檢測(cè)2萬多重要性

挖掘在《腫瘤致病基因檢測(cè)與轉(zhuǎn)移潛能分析》，發(fā)現(xiàn)收錄《Contrast Media Mol Imaging》在. 2022 Aug 16;2022:3188992.發(fā)表了一篇題目為《口腔腫瘤細(xì)胞外泌體 miR-10b 通過 AKT 信號(hào)傳導(dǎo)刺激細(xì)胞侵襲和遷移》腫瘤靶向藥物治療基因檢測(cè)臨床研究文章。該研究由Xiang Li, Ting Yang, Chuanji Shu 等完成。促進(jìn)了腫瘤的正確治療與個(gè)性化用藥的發(fā)展，進(jìn)一步強(qiáng)調(diào)了基因信息檢測(cè)與分析的重要性。

腫瘤靶向藥物及正確治療臨床研究?jī)?nèi)容關(guān)鍵詞：

口腔腫瘤,口腔癌細(xì)胞,外泌體,基因檢測(cè),靶向藥物

腫瘤靶向治療基因檢測(cè)臨床應(yīng)用結(jié)果

已鑒定出源自癌細(xì)胞的外泌體可調(diào)節(jié)細(xì)胞間通訊。然而，口腔癌衍生的胞外域在腫瘤轉(zhuǎn)移中的作用需要進(jìn)一步研究。我們?cè)诒疚闹醒芯苛怂鼈冊(cè)诳谇话┘?xì)胞中的作用。對(duì)口腔癌細(xì)胞的強(qiáng)制作用主要?dú)w因于 miR-10b，一種在外泌體中具有高水平的基因，通過口腔癌衍生的外泌體轉(zhuǎn)移到受體細(xì)胞。通過外泌體分離試劑獲得外泌體。此外，通過電子顯微鏡進(jìn)行外泌體鑒定和分析。通過qRT-PCR分析miRNA的表達(dá)。通過蛋白質(zhì)印跡分析蛋白質(zhì)表達(dá)。此外，還進(jìn)行了侵襲和遷移實(shí)驗(yàn)以測(cè)定和評(píng)估外泌體 miR-10b 的功能。根據(jù)研究結(jié)果，外泌體介導(dǎo)的 miR-10b 轉(zhuǎn)移促進(jìn)了口腔癌細(xì)胞的行為。賊后，發(fā)現(xiàn)AKT信號(hào)參與調(diào)節(jié)外泌體介導(dǎo)的口腔癌細(xì)胞侵襲和遷移，其激活降低了miR-10b敲低對(duì)口腔癌細(xì)胞的抑制作用。來自口腔癌細(xì)胞的外泌體 miR-10b 通過激活 AKT 信號(hào)傳導(dǎo)增強(qiáng)細(xì)胞侵襲和遷移。

腫瘤發(fā)生與反復(fù)轉(zhuǎn)移國(guó)際數(shù)據(jù)庫描述：

An exosome derived from a cancer cell has been identified to regulate intercellular communication. However, the roles of oral cancer-derived ectodomains in tumor metastasis need to be investigated further. We investigated their roles in oral cancer cells in this paper. The enforcing effect on oral cancer cells was attributed primarily to miR-10b, a gene with a high level in exosomes that is transferred to recipient cells via oral cancer-derived exosomes. Exosomes were obtained by exosome isolation reagents. Also, exosome identification and analysis were performed by electron microscopy. The expression of miRNAs was analyzed by qRT-PCR. Protein expression was analyzed by Western blot. Also, invasion and migration experiments were performed to assay and evaluate the function of exosomal miR-10b. Exosome-mediated transfer of miR-10b promoted oral cancer cell behaviors, according to the findings. Finally, it was discovered that AKT signaling participates in regulating exosome-mediated invasion and migration of oral cancer cells and its activation reduced the inhibitory effect of miR-10b knockdown on oral cancer cells. Exosomal miR-10b derived from oral cancer cells enhances cell invasion and migration by activating AKT signaling.