【佳學(xué)基因檢測(cè)】使用靶向序列捕獲增強(qiáng)病毒組測(cè)序

腫瘤基因檢測(cè)費(fèi)17800說(shuō)明

開(kāi)會(huì)學(xué)習(xí)醫(yī)學(xué)博士年度腫瘤匯報(bào)《腫瘤基因易感位點(diǎn)列表及發(fā)生率分析》《Sci Adv》在 2022 Mar; 8(9): eabm5386發(fā)表了一篇題目為《使用靶向序列捕獲增強(qiáng)病毒組測(cè)序》腫瘤靶向藥物治療基因檢測(cè)臨床研究文章。該研究由Igor Stevanovski, Data curation, Formal analysis, Investigation, Methodology, Resources, Software, Validation,  , Sanjog R. Chintalaphani, Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Software, Validation, Visualization, Writing - original draft, Writing - review & editing,  Hasindu Gamaarachchi, Formal analysis, Investigation, Methodology, Software, Validation, Writing - original draft,  James M. Ferguson, Formal analysis, Resources, Software,  Sandy S. Pineda, Conceptualization, Investigation, Methodology, Supervision, Visualization, Writing - review & editing,  ,  Carolin K. Scriba, Investigation, Resources,   Michel Tchan, Resources, Writing - review & editing,  Victor Fung, Investigation, Resources, Writing - review & editing,  Karl Ng, Resources, Validation, Writing - review & editing,  Andrea Cortese, Conceptualization, Investigation, Resources, Validation, Writing - review & editing,  ,  Henry Houlden, Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Methodology, Project administration, Resources, Software, Supervision, Validation, Visualization, Writing - original draft, Writing - review & editing,  ,  Carol Dobson-Stone, Investigation, Validation, Writing - review & editing,  Lauren Fitzpatrick, Investigation, Resources,  Glenda Halliday, Funding acquisition, Investigation, Resources, Supervision, Validation, Writing - review & editing,  Gianina Ravenscroft, Funding acquisition, Resources, Writing - review & editing,  Mark R. Davis, Resources,  Nigel G. Laing, Conceptualization, Funding acquisition, Investigation, Project administration, Resources, Supervision, Writing - review & editing,  ,  Avi Fellner, Writing - review & editing,  Marina Kennerson, Conceptualization, Resources, Validation, Writing - review & editing,   Kishore R. Kumar, Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Project administration, Resources, Supervision, Validation, Visualization, Writing - review & editing,   and Ira W. Deveson, Conceptualization, Data curation, Formal analysis, Funding acquisition, Methodology, Project administration, Supervision, Validation, Visualization, Writing - original draft, Writing - review & editing等完成。促進(jìn)了對(duì)人體內(nèi)及環(huán)境中未知病毒的快速和正確檢測(cè)，有利于更清晰地鑒定出疾病的發(fā)病原因。

病毒種屬鑒別及正確治療臨床研究?jī)?nèi)容關(guān)鍵詞：

宏基因組鳥(niǎo)槍法測(cè)序,MSS,靶向序列捕獲面板,ViroCap,一種計(jì)算方法

病毒種屬鑒別靶向治療基因檢測(cè)臨床應(yīng)用結(jié)果

宏基因組鳥(niǎo)槍法測(cè)序 (MSS) 是表征病毒種群的重要工具。它獨(dú)立于培養(yǎng)，不需要樣本中病毒的先驗(yàn)知識(shí)，并且可以提供有用的基因組信息。然而，MSS 可能缺乏敏感性，并且可能產(chǎn)生的數(shù)據(jù)不足以進(jìn)行詳細(xì)分析。我們創(chuàng)建了一個(gè)靶向序列捕獲面板 ViroCap，旨在從感染脊椎動(dòng)物宿主的 34 個(gè)家族的 DNA 和 RNA 病毒中富集核酸。一種計(jì)算方法將約 10 億 bp 的病毒參考序列壓縮成 <2 億 bp 的獨(dú)特的、具有代表性的序列，適用于靶向序列捕獲。我們比較了在標(biāo)準(zhǔn) MSS 中檢測(cè)病毒與靶向序列捕獲后的 MSS 中檢測(cè)病毒的有效性。首先，我們分析了兩組樣本，一組來(lái)自提交給診斷病毒學(xué)實(shí)驗(yàn)室的樣本，另一組來(lái)自兒童發(fā)燒研究中收集的樣本。我們?cè)趦山M中檢測(cè)到 14 和 18 種病毒，包括來(lái)自 10 個(gè)科的 19 個(gè)屬，在捕獲富集后顯著增強(qiáng)了基因組表示。捕獲后病毒讀數(shù)百分比的中位數(shù)增加倍數(shù)分別為 674 和 296。先進(jìn)組捕獲后的中位覆蓋廣度從 2.1% 增加到 83.2%，第二組從 2.0% 增加到 75.6%。接下來(lái)，我們分析了包含一組不同的指環(huán)病毒序列的樣本，并證明 ViroCap 可用于檢測(cè)與用于選擇捕獲探針的參考相比變異高達(dá) 58% 的病毒序列。 ViroCap 顯著增強(qiáng)了一套全面病毒的 MSS，并具有研究和臨床應(yīng)用的實(shí)用性。

腫瘤發(fā)生與反復(fù)轉(zhuǎn)移國(guó)際數(shù)據(jù)庫(kù)描述：

Metagenomic shotgun sequencing,MSS, targeted sequence capture panel, ViroCap, A computational approach